Insights into bacterial helicase loader mechanism and regulation

Insights into bacterial helicase loader mechanism and regulation

Loading mechanisms of ring helicases at replication

Mar 15, 2012 · Bin Liu, William K. Eliason, Thomas A. Steitz, Structure of a helicase–helicase loader complex reveals insights into the mechanism of bacterial primosome assembly, Nature Communications, 10.1038/ncomms3495, 4, (2013).

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Physical Basis for the Loading of a Bacterial Replicative

Apr 04, 2019 · In cells, dedicated AAA+ ATPases deposit hexameric, ring-shaped helicases onto DNA to initiate chromosomal replication. To better understand the mechanisms by which helicase loading can occur, we used cryo-EM to determine sub-4-Å-resolution structures of the E. coli DnaB⋅DnaC helicase⋅loader complex with nucleotide in pre- and post-DNA engagement states.

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Structural Mechanisms of Hexameric Helicase | F1000Research

Jan 27, 2016 · Although much is known about the loading and activation mechanism in the Gram-negative E. coli system, far less is known about SF4 helicases in the Gram-positive organisms where DnaI acts as the loader 65,125,126 or in bacteria which lack DnaC/DnaI loader homologs altogether 127,128.

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Structure of a helicase–helicase loader complex reveals

Sep 19, 2013 · During the assembly of the bacterial loader-dependent primosome, helicase loader proteins bind to the hexameric helicase ring, deliver it onto the oriC DNA and then dissociate from the complex.Here, to provide a better understanding of this key process, we report the crystal structure of the ~570-kDa prepriming complex between the Bacillus subtilis loader protein and …

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Physical Basis for the Loading of a Bacterial Replicative

Arias-Palomo et al. present the cryo-EM structures of a replicative bacterial helicase-loader complex (E. coli DnaBC) in pre- and post-loading states, revealing how the loader breaks the helicase ring to deposit it at the origin of replication and how ssDNA engagement closes and activates the helicase.

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Molecular mechanisms of transcription regulation by non

My research has provided important insights into their structure and function and, in the case of RECQL5, uncovered a novel mechanism of transcription regulation that might be employed by a number of other factors involved in transcriptional repression at the interface of the DNA recombination, replication and repair machineries.

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Loading mechanisms of ring helicases at replication

Mar 15, 2012 · Loading mechanisms of ring helicases at replication origins. Panos Soultanas. Corresponding Author. School of Chemistry, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK. E‐mail [email protected]

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The Eukaryotic Replication Machine - PubMed

Dec 01, 2012 · Structure of a helicase–helicase loader complex reveals insights into the mechanism of bacterial primosome assembly Bin Liu, 1, 2 William K. Eliason, 1, 2 and Thomas A. Steitz a, 1, 2, 3 1 Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA

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Structure of a helicase-helicase loader complex reveals

Jan 01, 2013 · Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly. Bin Liu 1] Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA [2] Howard Hughes Medical Institute, New Haven, Connecticut 06510, USA.

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Viral hijacking of a replicative helicase loader and its

Initiator and helicase regulation. By temporally separating helicase loading from activation, S-CDK can simultaneously activate the helicase while also inhibiting helicase loading factors to prevent origin re-firing.

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[PDF] Structure of a helicase–helicase loader complex

May 31, 2016 · To gain insights into both bacterial helicase loader mechanisms and how they may be disrupted by an exogenous viral system, we used a combination of structural, biochemical, and comparative studies to define how the phage 77 ORF104 protein inhibits S. aureus DnaI (SaDnaI). We show that the phage protein binds directly to DnaI's AAA+ ATPase …

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Loading mechanisms of ring helicases at replication

Mar 15, 2012 · Bin Liu, William K. Eliason, Thomas A. Steitz, Structure of a helicase–helicase loader complex reveals insights into the mechanism of bacterial primosome assembly, Nature Communications, 10.1038/ncomms3495, 4, (2013).

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DNA replication initiation: mechanisms and regulation in

Structure of a helicase–helicase loader complex reveals

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Frontiers | Single-Molecule Insights Into the Dynamics of

Aug 26, 2021 · Novel Insights Into Helicase Mechanisms Active Versus Passive Helicases. Ensemble biochemical studies have characterized replicative helicases and put forward the idea that these enzymes are universally poor motors when removed from their respective replisomes (Delagoutte and von Hippel, 2002; Delagoutte and von Hippek, 2003). The ensemble

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Engineering of a superhelicase through conformational control

Apr 17, 2015 · Conformational control of biomolecular activities can reveal functional insights and enable the engineering of novel activities. Here we show that conformational control through intramolecular cross-linking of a helicase monomer with undetectable unwinding activity converts it into a superhelicase that can unwind thousands of base pairs processively, even against a …

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Molecular insights into replication initiation in a

Molecular insights into replication initiation in a multipartite complex of replicative helicase DnaB and its loader DnaC (DnaB 6-DnaC 6) revisit the mechanism of oriC regulation as known in bacteria containing less than two copies of circular chromosome per cell. Mechanisms underlying the regulation of oriC function in

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Cdt1 revisited: complex and tight regulation during the

Oct 17, 2006 · Furthermore, deregulated Cdt1 induces chromosomal instability in normal human cells. Since Cdt1 is overexpressed in cancer cells, this could be a new molecular mechanism leading to carcinogenesis. In this review, recent insights into Cdt1 function and regulation in mammalian cells are discussed.

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Structure of a helicase–helicase loader complex reveals

Structure of a helicase–helicase loader complex reveals

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DNA replication initiation: mechanisms and regulation in

This Review presents an overview of both the mechanism and regulation of bacterial DNA replication initiation, with emphasis on the features that are similar in …

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New insights into replisome fluidity during chromosome

Helicase Primase SSB β clamp Pol III τ complex clamp loader RNA primer Lagging strand synthesis τ Pol α/primase C4 AND-1 Mcm 2-7 helicase ε RFC clamp loader Pol δ Cdc45 Mcm 10 RPA RNA primer Lagging strand PCNA Mrc1Claspin Leading strand TiBS Figure 1. Organization of proteins at a moving replication fork. (a) Bacterial replication fork.

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